番(fan)(fan)紅(hong)(hong)(hong)0的染(ran)(ran)色(se)(se)原理是(shi)(shi)嗜(shi)堿(jian)性(xing)的軟(ruan)(ruan)骨(gu)(gu)與堿(jian)性(xing)染(ran)(ran)料(liao)番(fan)(fan)紅(hong)(hong)(hong)O結(jie)合呈現紅(hong)(hong)(hong)色(se)(se)，嗜(shi)酸(suan)性(xing)的骨(gu)(gu)和酸(suan)性(xing)染(ran)(ran)料(liao)固(gu)綠(lv)結(jie)合而成綠(lv)色(se)(se)，與被染(ran)(ran)成紅(hong)(hong)(hong)色(se)(se)的軟(ruan)(ruan)骨(gu)(gu)對比鮮明，從而將軟(ruan)(ruan)骨(gu)(gu)組織和骨(gu)(gu)組織區分(fen)開(kai)。番(fan)(fan)紅(hong)(hong)(hong)O是(shi)(shi)一種結(jie)合多陰離(li)子的陽離(li)子染(ran)(ran)料(liao)，其(qi)顯示軟(ruan)(ruan)骨(gu)(gu)組織是(shi)(shi)基(ji)(ji)于(yu)陽離(li)子染(ran)(ran)料(liao)與多糖中(zhong)陰離(li)子基(ji)(ji)團（硫酸(suan)軟(ruan)(ruan)骨(gu)(gu)素或(huo)硫酸(suan)角質(zhi)素）結(jie)合。番(fan)(fan)紅(hong)(hong)(hong)O著色(se)(se)與陰離(li)子的濃度(du)(du)近(jin)似成正比關(guan)系，間接反(fan)映基(ji)(ji)質(zhi)中(zhong)蛋白多糖的含(han)量和分(fen)布(bu)。當軟(ruan)(ruan)骨(gu)(gu)受到損傷(shang)時(shi)，軟(ruan)(ruan)骨(gu)(gu)中(zhong)的糖蛋白會釋放出來，使基(ji)(ji)質(zhi)成分(fen)分(fen)布(bu)不均勻(yun)，從而導(dao)致(zhi)番(fan)(fan)紅(hong)(hong)(hong)O淡染(ran)(ran)或(huo)不著色(se)(se)。可(ke)通過圖像(xiang)分(fen)析軟(ruan)(ruan)件對番(fan)(fan)紅(hong)(hong)(hong)O染(ran)(ran)色(se)(se)的軟(ruan)(ruan)骨(gu)(gu)基(ji)(ji)質(zhi)進行定(ding)量分(fen)析。固(gu)綠(lv)與膠原纖維結(jie)合，不宜(yi)褪色(se)(se)。番(fan)(fan)紅(hong)(hong)(hong)O-固(gu)綠(lv)染(ran)(ran)色(se)(se)的分(fen)化很關(guan)鍵，分(fen)化過度(du)(du)易導(dao)致(zhi)切片(pian)不著色(se)(se)，分(fen)化不足易導(dao)致(zhi)切片(pian)著色(se)(se)過深。

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